Author(s):

Naved Ahmed Gauri and Rizwan Ahmed

Abstract: Plant growth promoting rhizobacteria (PGPR) are naturally occurring soil bacteria that aggressively colonize plant roots and can enhance plant growth by a wide variety of mechanisms. Bacteria are the most abundant among different rhizospheric microbes. Among Plant growth promoting bacteria, Bacillus is one of the most potential genera due to their spore forming ability, thereby increasing the adaptation of Bacillus strain to commercial formulation and field application In the present study, an attempt was made to isolate, screen and select different isolates of Bacillus and subjected to different test to identify their morpho-physiological, biochemical and In vitro characterization to study their plant growth promoting activities. The Rhizospheric soil samples were collected from different plants i.e. Triticumaestivum (wheat), Cynodondactylon (doobghas), Cicerarietinum (chana), Trifoliumamoenum (bersin) and from different locations of Dehradun and processed to isolate different isolates of Bacillus on nutrient agar medium. A total of 50 Bacillus isolates were isolated from the 22 soil samples. Interestingly, in the present study 17 isolates out of 50 were formed endospores, 5 isolates recorded as capsulated, 35 isolates were reduced the nitrate and all were recorded positive for IAA production. 6 isolates were found to be positive for siderophore production Biochemical characterization revealed that 12 isolates belonged to genus Bacillus belonging to five different species. Isolates NAB-1 seems to be B. alvei. Isolates NAB-8, NAB-11 and NAB-22 seem to be B. laterosporus. Isolates NAB-18, NAB-48, seems to be B. subtillis. Isolates, NAB-20, NAB-21 and NAB-25 seem to be B. larvae. Isolates NAB-23, NAB-43, NAB-44 seem to be B. alcalophilus.

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