Author(s):

Rizwan Ahmad, Gulnaz Parveen, Naved Ahmed Gauri and Dr. Neha Wal

Abstract: The present study was carried out to find out the antimicrobial activity of methanol extracts of three important medicinal plants respectively Putranjiva roxburghii, Achyranthus aspera and Citrullus colocynthis showed activity against all the selected bacterial strains. Methanol extracts of Achyranthus aspera and Citrullus colocynthis showed min. zone of inhibition against the selected gram positiveS. Haemolyticus (+ve) and maxi. Zone of Inhibition for gram negative bacteria K. pneumonia (-ve). Methanol extracts Putranjiva roxburghii of showed min. zone of inhibition against the selected gram positiveS. Haemolyticus (+ve) and maxi. Zone of Inhibition for gram positive bacteria B.cereus (+ve). Methanol extracts of three important medicinal plants respectively Putranjiva roxburghii, Achyranthes aspera and Citrullus colocynthis showed min. zone of inhibition against the selected gram positiveS. Haemolyticus (+ve). On comparing MIC concentration of different plant extracts with Standard Chloramphenicol (100μg/ml), it was observed that methanol extracts of Achyranthus aspera and Citrullus colocynthis gave best result against K. pneumonia (-ve), whereas Methanol extracts Putranjiva roxburghii gave best result against the selected gram positive (+ve)bacteria B. cereus. Antimicrobial analysis was done by using agar well diffusion method against bacterial pathogens. MIC value was determined by using micro broth dilution method. The phytochemical analysis showed the presence of tannins, flavonoids, saponins, terpenoids, and cardiac glycosides.

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