Author(s):

Chandan Haldar, Raju Ram, A Chaudhari, A Pavan Kumar and Gireesh Babu P

Abstract: Global aquaculture has expanded rapidly to address increasing demand for aquatic protein needs and an uncertain future for wild fisheries. To date, however, most farmed aquatic stocks are mostly wild and little is known about their genomes or the genes that affect important economic traits in culture. Many factors can influence individual disease resistance in target species but of particular importance in agriculture and aquaculture will be identification and characterization of the specific gene loci that contribute essential phenotypic variation to immunity because the information can be applied to speed up genetic improvement programs and increase productivity via marker-assisted selection (MAS). Genetic variation in a species improves the capability of organisms to adapt to changing environment and is vital for the survival of the species. The genetic diversity data have varied application in research on evolution, conservation, and management of natural resources and genetic improvement programs, etc. In our current study, we sequenced the different types of lectin genes using Sanger sequencing method to discover genetic diversity in Kerala wild population of Macrobrachium rosenbergii. We collected 23 individuals from Vembanad lake Kerala, and after sequencing the 23 gDNA, we found that a total of eleven SNPs in the three immune genes (Lectin3, lectin4, and tachylectin). We also reported three and two haplotypes from the LEC3 and LEC4 gene respectively, which shows the genetic diversity within the Kerala shock. The SNPs have considered as validated because all of them is detected through Sanger sequencing method. These SNPs could be used as a molecular marker in marker-assisted selection in the development of the QLTs for this species.

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