Author(s):

Ismot Ara, Roksana Sultana, Ismath Jahan Chanda and Nuhu Alam

Abstract: The current study was aim to isolate and indentify fungus associated with dried prawn and shrimp. Ovatospora brasiliensis, was identified through morphological characterization based on mycelium, conidia, colony features as well as molecular characterization. Polymerase chain reaction is remarkably effective at identifying species by genetic fingerprinting. The internal transcribed spacer (ITS) region of the ribosomal DNA (rDNA) of Ovatospora brasiliensis was amplified with the universal primers ITS4 and ITS5. Amplification of genomic DNA of Ovatospora brasiliensis length was 556bp. The clustering of the molecular data further revealed that the sequences represented fungal taxa at a cutoff of 99% molecular identity. These facts suggest the existence of enormous resources for molecular identification of fungal species based on ITS sequences. To the best of our knowledge, the experimental result concluded that dry shrimps contaminated with fungus caused by Ovatospora brasiliensis is first report in Bangladesh.

Pages: 43-47  |  1641 Views  526 Downloads

Download (641KB)