Author(s):

Samina Arif, Naim ul Hassan Naqvi, Muhammad Arshad Azmi, Abid Raza, Nadia Fatima, Sadaf Tabassum

Abstract: Toxicity of Deltamethrin essential oil against Callosobruchus analis by three methods Direct Application method (DAM), Glass film method (GFM) and Filter paper impregnation method (FIM) after 24 hours of treatment. The LC50 value calculated as 0.3352 µl/cm2 by DAM, 0.043901 µl/cm2 by GFM and 0.03472 µl/cm2 by FIM. HPLC analysis shown the activity of Deltamethrin (standard) against C. analis by (DAM) shown by peaks 89765, 6785, 3365 and in Deltamethrin (sample) peaks results shown as 79918, 2672, 1348 µm, similarly by (GFM) Deltamethrin (standard) shown as one peak 7449, peak (2) and (3) were absent and Deltamethrin (sample) observed as 89885, 10689, peak 93) was absent, similarly by (FIM) Deltamethrin (standard) found as one peak 59784, peak (2) and (3) were absent and Deltamethrin (sample) shown as two peaks which was 73809, 2115 µm.

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