Author(s):

Ashok Kumar Chaubey, Istkhar, Aashana, Aashaq Hussain Bhat, Aasha

Abstract: Present study deals with the identification of an isolate of Heterorhabditis indica CH₁₆ of District Bijnor, Uttar Pradesh, India with high virulent and reproductive potential grounded on morphotaxometry and multigene sequence analyses. Total body length (520 µm vs. 528 µm) support the close similarity of specimen with H. indica which was greater to H. tysesare (418 µm) and shorter with others. With a length of 547 µm, males of present isolate were shortest. Results obtained through the molecular phylogeny of sequences of ITS rDNA, and 28S rDNA placed the isolate CH₁₆ with H. indica and H. noenieputensis. However analysis of COXI gene revealed unsatisfactory results with diverting results. Bioassay trial against Galleria mellonella showed greater pathogenicity of nematode isolate where it killed all the larvae within 60 hours post infection period with all the doses applied. Increment in dose lead to the increased number of progeny was recorded up to 100 IJs/ Larva (2.15 lacs IJs/ Larva) which was higher when compared to others. Results demonstrated that the multi-sequence analyses provide more reliable and accurate data for the diagnostic of Heterorhabditis species. Further, the high virulence and reproductivity prove that the present isolate as good candidate for future IPM program.

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