Kituyi Christine Nasimiyu, Ndeti Cosmas Muia, Maina Geoffrey, Mongâ€™are Samuel, Ingonga Johnstone, Nyambati Venny, Anjili Christopher Omukhango
water extracts have antileishmanial activities. In thernpresent study, the efficacy of combination therapy using A
against L. major
was studied bothrnintraperitoneally and orally. The standard drug pentostam and phosphaternbuffered saline were used as positive and negative controls. T-test and ANOVArnwere used for data analysis and P-value of < 0.05 was consideredrnsignificant. Plant materials were dried, ground soaked in water at 80 o
Crnfor 1 hour, filtered then freeze dried. The minimum inhibitory concentrationsrnof aqueous extracts of A. secundiflora
and A. sativum
g/ml and 5000 Î¼
g/ml and IC50
g/ml respectively. The IC50
for their combination atrnratio (1:1) was 391.79 Î¼
g/ml as compared to minimum inhibitoryrnconcentrations of 12.5Î¼
g/ml and IC50
g/ml forrnpentostam. The combination therapy had Infection rate of 17% and multiplicationrnindex of 48.65% compared to pentostam (IR=21% and MI=11.64%). The combinationrntherapy reduced the footpad lesion size significantly (P
<0.05) likernthe pentostam control drug and no significant nitric oxide stimulated. The oralrnand intraperitoneal combination treatment reduced spleen amastigotes in mice byrn55.48% and 64.13% corresponding to total LDUs of 18.23Â±0.90and 14.69Â±1.33rnrespectively compared to pentostam 94.58% and LDU of 2.22Â±0.13. The combinationrntherapy was less toxic, effective against L. major
parasite, reducedrnlesion size significantly, reduced spleen parasite load significantly but didrnnot prevent visceralization.
Kituyi Christine Nasimiyu, Ndeti Cosmas Muia, Maina Geoffrey, Mongâ€™are Samuel, Ingonga Johnstone, Nyambati Venny, Anjili Christopher Omukhango. In vitro and in vivo efficacy of combination therapy using Allium sativum and Aloe secundiflora against Leishmania major infected BALB/c mice. Int. J. Fauna Biol. Stud. 2016;3(2):102-108.