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International Journal of Fauna and Biological Studies

FAUNA

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e-ISSN : 2347-2677, p-ISSN : 2394-0522

International Journal of Fauna and Biological Studies

International Journal of Fauna and Biological Studies

2017, Vol. 4 Issue 5, Part B

DNA Barcoding of local pigs in minahasa, north sulawesi

Author(s):

Revolson Alexius Mege and Mokosuli Yermia Semuel

Abstract:
DNA barcoding and Molecular phylogeny reconstruction of local pigs from Minahasa North Sulawesi, Indonesia was investigated using partial sequences of Cytochrome c oxidase subunit I gene (CO1). Two location according the place of traditional pigs farm used this study that is Minahasa Utara and Minahasa was analysis the partial CO1 gene. Phylogeny trees was constructed online in NCBI site. From the results of this studied, based on partial CO1 gene, local Pigs of Minahasa cannot be sure of the position of species, because it does not have a sequence similar to the gene bank. The North Minahasa local pigs showed the similarity of the nearest CO1 sequence to the Pig DNA sequence from clone WTSI_1061-78D9. Similarly, the phylogeny construction shows the closest kinship based on the CO1 gene with Pig DNA sequence from clone WTSI_1061-78D9.

Pages: 82-87  |  946 Views  133 Downloads

How to cite this article:
Revolson Alexius Mege and Mokosuli Yermia Semuel. DNA Barcoding of local pigs in minahasa, north sulawesi. Int. J. Fauna Biol. Stud. 2017;4(5):82-87.
International Journal of Fauna and Biological Studies