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International Journal of Fauna and Biological Studies

FAUNA

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Impact Factor: RJIF 5.53

e-ISSN : 2347-2677, p-ISSN : 2394-0522

International Journal of Fauna and Biological Studies

International Journal of Fauna and Biological Studies

2017, Vol. 4 Issue 5, Part B

Morphological and cytochemical characterization of the sternal leukopoietic cell lineages in wistar rat

Author(s):

Damir Suljevic, Alen Hamzic, Muhamed Focak, Amir Zahirovic and Andi Alijagic

Abstract:
This research provides the first data on the morphological identification of the leukopoietic lineages (leukopoies) in the bone marrow of the Wistar rats. Seven types of cells were identified: myeloblasts, promyelocytes, myelocytes (neutrophils, eosinophils and basophils), metamyelocytes and band granulocytes. It was necessary to apply standard staining by using Pappenheim and cytochemical peroxidase method protocol for the proper cellular identification. Because of the consistency of the tissue, bone marrow isolation is not possible by the standard methods, it is only possible by the use of the touch technique. Mature neutrophils had high prevalence in the bone marrow, followed by the neutrophilic myelocytes. The largest among observed cells were the myeloblasts and promyelocytes. Identified cells showed great resemblance to human bone marrow cells, which is very important for any future experimental research.

Pages: 99-103  |  800 Views  43 Downloads

How to cite this article:
Damir Suljevic, Alen Hamzic, Muhamed Focak, Amir Zahirovic and Andi Alijagic. Morphological and cytochemical characterization of the sternal leukopoietic cell lineages in wistar rat. Int. J. Fauna Biol. Stud. 2017;4(5):99-103.
International Journal of Fauna and Biological Studies