International Journal of Fauna and Biological Studies
2019, Vol. 6 Issue 6, Part A
Profile of estrogen and progesteron hormones in bali cows that are exposed in the natural grazing filed
Ambius Anton, Nyoman Sadra Dharmawan, I Gede Mahardika, Wayan Bebas, I Gusti Ngurah Bagus Trilaksana and I Gusti Nyoman Gde Bidura
The reproductive process in productive cows, controlled by hormones, and physiological events is important to understand in improving reproductive efficiency. The study aims to determine the hormone profile of estrogen and progesterone in bali cow during one estrous cycle. The study used 10 bali cattle aged 3-4 years, Body Condition Scor (2-3), not breastfeeding, and in the luteal phase. Cows were synchronized with estrus by injecting 25 mg PGF2a (Dinoprost) intramuscularly. Detection of the emergence of estrus is done twice a day, namely: morning and evening after being treated. Blood collection is carried out on day 0 (during estrus), 3rd, 9th, 15th, and 17-23th to determine the levels of the hormones estrogen and progesterone. Measurement of estrogen (pg/ml) and progenteron (ng/ml) levels by the method of Enzyme Linked Immunoabsorbant Assay (ELISA). The results showed that the appearance of estrus in cows was 3.40Â±0.52 days after PGF2Î± injection, with an estrous cycle length of 22 days. Estrogen hormone levels on days 0, 3, 9, 15, and 17-23 were: 56.97-1.19; 16.40Â±0.40; 21.89Â±1.02; 15,37Â±0,37; 17.87Â±0.52; 26,32Â±0.43; 31.31Â±0.82; 44.01Â±0.83; 53.74Â±0.96; 55.71Â±0.65; 53.94Â±0.77 pg/ml, respectively and levels of the hormone progesterone on days 0, 3rd, 9th, 15th, and 17-23 were: 0.35Â±0.10; 1.35Â±0.72; 5.07Â±0.15; 7.15Â±0.08; 6.24Â±0.13; 5.69Â±0.04; 4.67Â±0.05; 3.20Â±0.03; 1.18Â±0.05; 0.43Â±0.02; 0.43Â±0.03 ng/ml, respectively. It can be concluded that the levels of estogenic hormones at the time of estrus were highest (56.97Â±1.19 pg/ml and 55.71Â±0.65) with basal progesterone levels (0.35Â±0.10 and 0.43Â±0, 03 ng/ml).
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Ambius Anton, Nyoman Sadra Dharmawan, I Gede Mahardika, Wayan Bebas, I Gusti Ngurah Bagus Trilaksana and I Gusti Nyoman Gde Bidura. Profile of estrogen and progesteron hormones in bali cows that are exposed in the natural grazing filed. Int. J. Fauna Biol. Stud. 2019;6(6):28-33.