Background: Plasmodium infection (Malaria) always remains hazardous for its fatalities worldwide, especially for Pakistan; it is yet endemic in many areas. Out of five types of Plasmodium, Plasmodium vivax and Plasmodium falciparum is dominating in Pakistan. P. vivax is more frequent and widely distributed while P. falciparum is more dangerous and deadly in action. This research was conducted for the assessment of Plasmodium infection frequency in human populations of District Multan, Punjab, Pakistan.

Methods: Plasmodium type was identified in the blood of high graded fever patients. Immuno chromatographic Technique (ICT) as well as thick and thin blood smears procedure was performed at different laboratories of district Multan where the microscopic examination was carried out for the presence of Plasmodium types and its species.

Results: Statistically, the current prevalence and distribution of Plasmodium types was estimated in this study. A total of 192 blood samples were examined. The Plasmodium species recovered were P. vivax, P. falciparum and mixed species. The prevalence of P. vivax, P .falciparum and mixed species was 13.02%, 10.41% and 1.041% respectively.

Conclusion: Plasmodium vivax had prevalence over Plasmodium falciparum. The prevalence species was more in males as compared to females. The age-wise prevalence of plasmodium infection showed that it was more prevalent in patients of age group 21-30 years.