S Kannan, Vasuki Vivekananda, Lokesh Makam and SP Venkatesh Prasad

A comparative study of chemo-detection ability to stimulate the pheromone-responsive neurons (PRNs) was conducted to evaluate the red palm wee vil (RPW) antennal olfactory sensillum response to elicit depolarization of olfactory sensory neurons (OSNs) producing optimal neuronal action potential. Electroantennography (EAG) was carried out using insect antenna and corresponding electroantennograms were recorded for selected different aggregation pheromone blends of variable ratios, results revealed that aggregation pheromones 4-methyl-5-nonanol and 4-methyl-5-nonanone, in combination produced distinct electroantennograms establishing chemo-detection ability by the insect. The experimental results demonstrated chemo-detection ability by the antennal olfactory sensillum of RPW, producing optimal neuronal response by weevil for the blend of 4-methyl-5-nonanol and 4-methyl-5-nonanone at 7:1 blend ratio and was found to be the optimum blend ratio to produce good action potential and depolarization effect.

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