Olusola Sokefun

This current study assessed the population of Parachanna obscura from the Lagos lagoon. It is the first study that is examining the usefulness of DNA barcoding in identifying the Lagos Lagoon in Nigeria populations and also shedding more light on their taxonomic status. The CO1 gene yielded consistently 654 base pairs after alignment. Intraspecific divergence is low ranging from 0.00 to 0.05, while interspecific divergence with other available gene sequences is between 0.00 to 0.2. Average nucleotide composition were in the order T > C > A > G (T: 29.1, C: 28.7, A: 22.1 and G: 20.0). There were 648 conserved sites and 4 variable sites. One hundred and five (105) of the sites were polymorphic, with One Hundred and twenty One (121) mutations and a nucleotide diversity of 0.0937, Tajima’s diversity of 2.52 at a statistical significance of p<0.05 was also observed. There were fourteen (14) unique haplotypes. The Lagos lagoon population all mapped together with a bootstrap value of 99% indicating some level of uniqueness, even though their genetic diversity is very low. Further research will be required to add more sampling sites around the Lagos lagoon and also more genetic markers with deeper analysis to see if there are population cryptics.

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