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International Journal of Fauna and Biological Studies

FAUNA

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e-ISSN : 2347-2677, p-ISSN : 2394-0522

International Journal of Fauna and Biological Studies

International Journal of Fauna and Biological Studies

2023, Vol. 10 Issue 4, Part A

Evaluation of gelatin characterization of Bali cattle hide based on the FTIR approach and molecular weight

Author(s):

I Nyoman Sumerta Miwada, I Ketut Sumadi, Putu Wrasiati and I Nyoman Sutarpa Sutama

Abstract:

The aim of this research is to develop gelatin from protein extraction from bali cattle hide and to evaluate its conformation through the FTIR approach and the molecular weight of gelatin. The research method used a completely randomized design with a factorial pattern (4 x 3), namely acetic acid (A1=2%; A2=4%; A3=6% and A4=8%) and curing time (C1=2 days; C2= 4 days and C3 = 6 days). Each treatment was repeated 3 times. The results showed that the results of the FTIR test, gelatin from the extraction of collagen protein from the hide of bali cattle tested positive as a protein and was identified as a hydroxyl, carbonyl and amide group. The FTIR test results are in the frequency range (cm-1) between 500 – 3500. Meanwhile, the molecular weight study using the SDS-PAGE method is predicted to range from 67-135 kDa. The conclusion of the study is that the effect of using different concentrations of acetic acid and different curing times has not given a different spectrum of changes to collagen protein in bali cattle hide. Meanwhile, a high concentration of acetic acid and a longer curing time causes shorter protein degradation by producing a lower molecular weight.

Pages: 18-23  |  207 Views  81 Downloads

How to cite this article:
I Nyoman Sumerta Miwada, I Ketut Sumadi, Putu Wrasiati and I Nyoman Sutarpa Sutama. Evaluation of gelatin characterization of Bali cattle hide based on the FTIR approach and molecular weight. Int. J. Fauna Biol. Stud. 2023;10(4):18-23. DOI: 10.22271/23940522.2023.v10.i4a.969

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