Faria Farhana Rain and Abu Faiz Md. Aslam

Recently, DNA barcoding has become an effective method for the accurate identification of species. This work is the first attempt to identify the bed bug species based on the MT-COI gene from Bangladesh. Nucleotide composition analysis revealed that A-T base content was higher than G-C base contents in mtDNA of C. hemipterus. The intraspecific genetic divergence range of C. hemipterus was 0.000-0.002. Phylogenetic analysis revealed that all sequences of C. hemipterus were clustered in the same major clade. Single nucleotide polymorphism was found in 53 positions of nucleotide sequences of C. hemipterus_4. Therefore, our results suggest that COI barcodes can contribute to the exact identification of bed bugs which can aid in pest management programs.

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