Dr. Ashutosh Tripathi

The black garden ants Lasius niger are a major agricultural and urban pest, destroying crops and invading residential areas both outdoors and indoors. 3-5 mm long, workers are dark glossy black. As the colony gets older it has been known for workers to increase in size over generations. Aqueous extracts of varying concentrations (100%, 75%, 50%, 25%, 10%, 5%, 1% and 0.1%) of fresh leaves of Annona squamosa were sprayed over workers of Lasius niger. The dilution calculated by the formula weight per volume, with the solvent RO Water. While mMortality is defined as dead /number of subjects x 100%. We used positive control group that was exposed with the insecticide Chlorpyriphos 2% and a negative control group which is exposed by RO Water. The leaves extract from Annona squamosa with Extract leaves 25%, 50%, 75% and 100% (w/v) concentration Annona squamosa showed effectively kill Lasius niger, but less effective if compared to the positive control insecticide Chlorpyriphos 0.2% while extract leaves 0.1%, 5%, and 10% ineffective. While, LD50 of the extract was very high (61.30%).

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