Author(s):

Ujjwala Shivaji Deshmukh

Abstract: Study was intended to assess antimicrobial activity of silk of spider Nephila pilipes and Stegodyphus sarasenorum on bacteria. Silk extracts were prepared separately in three different solvents Acetone, Ethanol and Methanol. Zone of inhibition was observed for bacteria E. coli after 24 hours. Apparent inhibition of bacterial growth was observed. For E. coli maximum zone of inhibition observed with silk extract of N. pilipes in Methanol extract 18 mm, followed by Acetone extract shown 14 mm diameter and 10 mm antimicrobial activity was found in Ethanol extracts. Maximum zone of inhibition observed with S. sarasenorum silk extract in Acetone 14 mm, Methanol 12 mm and Ethanol extract has shown the diameter 6 mm .

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