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International Journal of Fauna and Biological Studies

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e-ISSN : 2347-2677, p-ISSN : 2394-0522

International Journal of Fauna and Biological Studies

International Journal of Fauna and Biological Studies

2017, Vol. 4 Issue 2, Part B

Phylogeny reconstruction of Gerridae from River Ratatotok and River Talawaan North Sulawesi Based on Mitochondrial DNA 16 S RNA Gene

Author(s):

Danny Waha, Max Tullung, Siegfried Berhimpon and Feky Mantiri

Abstract:
Has conducted research that aims to get the position species based Gen 16 S RNA Mitochondrial DNA of Water Striders in Talawaan River and Ratatotok River, North Sulawesi. Extraction of total DNA using DNA Innuprep Micro KIT (Analytic Jena, Germany). 16 S RNA gene [16sA (CTG 5'CGC AAA TTT AAC AAC AT 3'); 16sB2 (5'TTT AAT ACA TCG AGG CCA 3')]. 16 S RNA gene amplification using Master Cyler with Rotor Gene Q Series Software 2.0.3. PCR conditions that denaturation initiation stage 94 0C for 3 minutes, denaturation stage 94 0C for 30 seconds, annealing step 52 0C for 50 seconds, extension stage 72 0C for 1 min and final extension 72 0C for 5 minutes. Visualization amplicon using Automatic Electrophoresis Qiaxel. Sequencing was performed using the ABI PRISM 3730xl Genetic Analyzer Develop by Applied Biosystems, USA. Construction phylogeny using MEGA 6.0 program. Based on the phylogeny tree formed from Talawaan Water striders have closest kinship with Limnoporus esakii while Water striders of Ratatotok has the closest kinship with Cylindrostethus costalis.

Pages: 81-87  |  818 Views  37 Downloads

How to cite this article:
Danny Waha, Max Tullung, Siegfried Berhimpon and Feky Mantiri. Phylogeny reconstruction of Gerridae from River Ratatotok and River Talawaan North Sulawesi Based on Mitochondrial DNA 16 S RNA Gene. Int. J. Fauna Biol. Stud. 2017;4(2):81-87.
International Journal of Fauna and Biological Studies