International Journal of Fauna and Biological Studies
2020, Vol. 7 Issue 3, Part A
Sequencing and phylogenetic melanocortin-1-receptor (MC1R) gene of bali cows in kupang district, nusa tenggara timur provincy of Indonesia
A.C Tabun, Sumadi, C.L Leo Penu, J.A Jermias, S.D Volkandari, T Hartatik and I Gusti Nyoman Gde Bidura
Bali cattle is a type of beef cattle that must be preserved and developed because Bali cattle are Indonesian germplasm that belong to the genetic resources of local beef cattle. The research objectives is to determine to study the nucleotide sequence and phylogenetic tree melanocortin 1 receptor (MC1R) gene of different coat color in Bali cows of Kupang. Blood samples were taken from seventeen sorrel, eighteen black, and eight white of Bali cows, respectively. The method used research was PCR and DNA sequencing. The amplification of the PCR product to the MC1R-gene of Bali cows in Kupang was at 296bp. Nucleotide change were c.52C>T, c.223C>A in cattle (sorrel, and white Bali cows) and c.147C>T in cattle (black; sorrel and white Bali cows). Nucleotide C plays an important role in the formation of black pigment, changes nucleotide C to nucleotide T causes a change in coat color at sorrel and white Bali Cows. It can be concluded that the melanocortin 1 receptor gene play a role in the formation of coat color in Bali cow Kupang with a percentage of nucleotide similarity were 99.662% at black Bali cow, Sorrel and white Bali cows were 98.986%.
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A.C Tabun, Sumadi, C.L Leo Penu, J.A Jermias, S.D Volkandari, T Hartatik and I Gusti Nyoman Gde Bidura. Sequencing and phylogenetic melanocortin-1-receptor (MC1R) gene of bali cows in kupang district, nusa tenggara timur provincy of Indonesia. Int. J. Fauna Biol. Stud. 2020;7(3):01-06.